Influence of poly(ADP-RIBOSE) polymerase inhibitors on some parameters of oxidative stress in blood leukocytes of rats with experimental diabetes

Abstract

The study was undertaken to investigate the influence of specific inhibitors of poly(ADP-ri-bose)polymerase-l (PARP-1), in particular nicotinamide and 1,5-isoqinolinediol on white blood cells of rats with diabetes. Using the fluorescent probe 2′,7′-dichlorodihydrofluorescein diacetate ROS production in leukocytes was asseced. It was found that the development of streptozotocin-in-duced diabetes was accompanied by an intensification of oxidative stress and a significant decrease in viability of blood leukocytes as compared to control animals. Administration of PARP-1 inhibitors prevented the development of oxidative stress in leukocytes and increased their viability. It was shown a reduction of superoxide dismutase activity in serum in diabetes. Investigated PARP-1 inhibitors had no effect on the activity of superoxide dismutase and glucose levels in the blood. The findings suggest the intensification of oxidative stress in leukocytes of diabetic animals and the ability of nicotinamide and 1,5-isoqinolinediol to prevent its development depending on the features of their structure.