Evaluation of disposable microfluidic chip design for automated and fast Immunoassays

Abstract

We report here, the design and development of a disposable immunoassay chip for protein biomarker detection within -1 h. The unique design allows for realtime dynamic calibration of immunoassay for multiple biomarker detections on the chip. The limit of detection achieved for this test chip is 10 pg/ml for IL6, 50 pg/ml for GFAP with a detection time of 1 h. The prototype instrument used for flowing the reagents through the chip can be easily assembled from off-theshelf components with the final chemiluminescent detection carried out in a commercial plate reader. Optimization of different aspects of chip design, fabrication, assay development is discussed in detail.