Evaluation of potential probiotics properties of the screened Lactobacilli isolated from home-made koumiss in Mongolia

Abstract

Among thirty Lactobacillus strains, which were isolated and identified from the home-made koumiss in Mongolia, Lactobacillus helveticus MG2-1 was screened out for the best acid tolerance in artificial gastric juice at pH 3.0 after incubating for 3 h with the survival rate 96.15 ± 4.22%. Further in vitro assessment revealed MG2-1 showed high tolerance to the artificial gastrointestinal juice and bile salts. Moreover, other desired probiotic properties, including the ability of sodium taurocholate deconjugation (with the amount of cholic acid liberated 2.95 ± 0.11 μM), cholesterol removal (cholesterol removal rate 51.74 ± 0.04%) and adhesion to Caco-2 cells (the adhesive rate 1.51 ± 0.11%), were also observed. The results highlighted the probiotic potential of MG2-1, which deserved further investigation in in vivo studies to elucidate its health benefits.