The optimization of eluting condition of solid phase extraction method for α-mangostin purification in mangosteen pericarp extract

Abstract

Objective: To obtain the optimum condition of eluent selection for purification of α-mangostin in mangosteen pericarp extract with solid phase extraction (SPE) method. Methods: The analysis was conducted by SPE cartridge octadecylsilane (ODS)-5, solvents used were methanol for conditioning, aqua bidestilated for washing and three different eluents for eluting: methanol, ethanol and ethyl acetate. Results: Recovery value for each eluent were 32.4741% for methanol, 46.6130% for ethanol and 33.6383% for ethyl acetate. Comparison of alpha-mangostin level in the mangosteen pericarp extract for each eluent i.e. 14.4578% for methanol, 10.0598% for ethanol, and 14.2898 % for ethyl acetate while compared to the extract without SPE was 21.7934%. Conclusion: Ethanol had the highest recovery value, compared to methanol and ethyl acetate.