Trans-splicing of mRNAs links gene transcription to translational control regulated by mTOR

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Abstract

Background: In phylogenetically diverse organisms, the 5′ ends of a subset of mRNAs are trans-spliced with a spliced leader (SL) RNA. The functions of SL trans-splicing, however, remain largely enigmatic. Results: We quantified translation genome-wide in the marine chordate, Oikopleura dioica, under inhibition of mTOR, a central growth regulator. Translation of trans-spliced TOP mRNAs was suppressed, consistent with a role of the SL sequence in nutrient-dependent translational control of growth-related mRNAs. Under crowded, nutrient-limiting conditions, O. dioica continued to filter-feed, but arrested growth until favorable conditions returned. Upon release from unfavorable conditions, initial recovery was independent of nutrient-responsive, trans-spliced genes, suggesting animal density sensing as a first trigger for resumption of development. Conclusion: Our results are consistent with a proposed role of trans-splicing in the coordinated translational down-regulation of nutrient-responsive genes under growth-limiting conditions.

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Danks, G. B., Galbiati, H., Raasholm, M., Torres Cleuren, Y. N., Valen, E., Navratilova, P., & Thompson, E. M. (2019). Trans-splicing of mRNAs links gene transcription to translational control regulated by mTOR. BMC Genomics, 20(1). https://doi.org/10.1186/s12864-019-6277-x

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