DNA Amplification Techniques

Abstract

The polymerase chain reaction (PCR) has revolutionized modern science. With the introduction of the PCR to the clinical laboratory, genetically based diseases are now diagnosed with an incredible level of sensitivity and accuracy. This chapter will review the original discovery of the PCR in which the synthetic cycles were driven by the Klenow firagment of DNA polymerase I, and the rapid development of this exciting technology after the introduction of the heat-resistant Thermus aquaticus (Taq) polymerase.