The determination of partition coefficient of 6-mercaptopurine derivatives by thin layer chromatography

Abstract

Mercaptopurine and its derivatives are used in the treatment of leukemia. To estimate their lipophilicity, a simple and novel thin layer chromatography method was developed. The mobile phase was the mixture of acetonitrile and water. The acetonitrile content varied by 5 from 50 to 80. The linear relationship between log P and R m 0 for substances with known lipophilicity was found. The lipophilicity of purine derivatives was worked out from the calibration curve. The most lipohilic compound was methylazathioprine (0.64). © 2013 Andrzej Czyrski and Bartomiej Kupczyk.