ROLE OF LACTOFERRIN ON THE HISTOPATHOLOGICAL CHANGES OF HYPERLIPIDEMIC-INDUCED FATTY LIVER IN RATS

Abstract

Introduction: Hyperlipidemia is a condition that may lead to many illnesses. The worldwide prevalence of hyperlipidemia is still relatively high. Lactoferrin is a glycoprotein found in mammals and fishes’ epithelial mucosa cells, also known for its protective roles. Objective: This study aims to discover the histopathologic changes in rat’s liver after the intervention of 100, 200, and 400 mg/kgBW lactoferrin. Method: This study involved 30 Sprague-Dawley rats divided into six groups: normal, negative control, statin-treated, dose 1, dose 2, and dose 3. The normal group was given standard feed, while the other groups were hyperlipidemic induced by a high cholesterol diet. The intervention for the statin-treated group was 1.5 mg/kgBW statin, while dose 1, 2, and 3 were respectively given 100 mg/kgBW, 200 mg/kgBW, and 400 mg/kgBW of lactoferrin. After six weeks, all rats were dissected, and the livers’ histopathologic slides were scored with Manja Roenigk scoring. Result: Lactoferrin improved the fatty liver condition. The statistical test results showed that 200 mg/kgBW of lactoferrin caused a significant change in the rats’ liver structures (p < 0.05). Conclusion: 200 mg/kgBW of lactoferrin significantly improved the histopathologic structure of fatty liver.