Isolation of Genomic DNA from Mammalian Cells and Fixed Tissue

Abstract

Examination of DNA variation is central to understanding the function of mammalian cells, tissues, and whole bodies. Extraction of high-quality DNA from cells and tissues is necessary for innumerable different experiments. We present protocols for the extraction of DNA from both fresh samples and formalin-fixed tissue. Methods for extracting DNA have been standardized and streamlined over the past couple of decades and many extraction kits are available for a reasonable cost. In addition, many of the extraction procedures can also be automated for even higher throughput sample preparation. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: DNA isolation from whole blood, tissue, and cultured cells. Alternate Protocol: DNA extraction using automated machines. Basic Protocol 2: DNA isolation from saliva and buccal swabs. Basic Protocol 3: DNA isolation from formalin-fixed, paraffin-embedded tissue.