Cell-based assay by immunofluorescence cytochemistry (CBA-IFC) has been shown to be the most accurate method to detect anti-aquaporin (AQP) 4 autoantibodies. Detection of anti-AQP5 autoantibodies is delicate, which depends on the proper expression of AQP5 on the plasma membrane. Here, we describe methods to determine anti-AQP5 autoantibodies by CBA-IFC. Both anti-AQP5 IgG and IgA can be detected by this method.
CITATION STYLE
Alam, J., Jeon, S., & Choi, Y. (2019). Determination of anti-aquaporin 5 autoantibodies by immunofluorescence cytochemistry. In Methods in Molecular Biology (Vol. 1901, pp. 79–87). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8949-2_6
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