A Novel Computer Assisted Sperm Analyzer for Assessment of Spermatozoa Motility in Fish; BASA-Sperm Aqua

Abstract

This study was conducted to determine the working principle and operability of the BASA-Sperm Aqua module software of the newly developed computer-assisted sperm analysis system (BASA) for the evaluation of spermatozoa motility in fish. Semen samples of trout (Oncorhynchus mykiss) species were examined for this purpose. Sperm motility parameters such as VSL (μm/s), VCL (μm/s), VAP (μm/s), LIN (%), BCF (Hz), ALH (μm) and MAD (o) were examined. The investigated parameters were compared with data which analyzed in similar computer systems and published in international manuscripts. Finally, the BASA-Sperm Aqua has been found to be a software that performs its functions very quickly and practical and produces accurate and understandable results in the determining sperm quality parameters of fish.