Preparation and evaluation of xylose palmitate ester using candida antarctica lipase b as a reusable biocatalyst

Abstract

THE direct activity enzymatic and esterification stability of lipase of D-xylose B from with Candida palmitic antarctica acid using were ethylmethylketone investigated in as reaction media. The xylose palmitate was obtained under the optimized conditions, 1:1 molar ratio of xylose: palmitic acid, at 250 rpm and 60°C. Effect of the use of Butanol to wash the immobilized lipase before the enzyme reuse was evaluated. The lipase could be successfully reused four times without loss of activity. Various physicochemical characteristics of 1-O-hexadecanoyl-D- xylopyranose were also studied.