An enrichment procedure has been developed that results in at least a 200 X increase in mutation frequency in the colonial alga, Eudorina elegans. A period of nitrogen starvation followed by treatment with 8-azaguanine results in the death of wild-type cells and the maintenance of mutants. N'-nitro-N-nitro-soguanidine-induced acetate, p-aminobenzoic acid and reduced nitrogen requiring mutants have been isolated by this procedure.
CITATION STYLE
Toby, A. L., & Kemp, C. L. (1975). Mutant enrichment in the colonial alga, Eudorina elegans. Genetics, 81(2), 243–251. https://doi.org/10.1093/genetics/81.2.243
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