A mutant from Escherichia coli which lacks ribosomal proteins S17 and L29 used to localize these two proteins on the ribosomal surface

Abstract

A mutant of Escherichia coli has been isolated which lacked ribosomal proteins S17 and L29, as judged by two‐dimensional gel electrophoresis. A battery of immunological tests was used to confirm this result. Ribosomes of this mutant were used as a control for the localization of proteins S17 and L29 on the surface of the ribosomal subunits of E. coli. Protein S17 has been localized on the 30S subunit body, 3–5 nm away from the lower pole, while protein L29 is located at the back of the 50S particle on the opposite side to the interface. Copyright © 1985, Wiley Blackwell. All rights reserved