Novel activity-based probes for broad-spectrum profiling of retaining β-exoglucosidases in situ and in vivo

Abstract

A high-end label: Cyclophellitol aziridine-type activity-based probes allow for ultra-sensitive visualization of mammalian β-glucosidases (GBA1, GBA2, GBA3, and LPH) as well as several non-mammalian β-glucosidases (see picture). These probes offer new ways to study β-exoglucosidases, and configurational isomers of the cyclophellitol aziridine core may give activity-based probes targeting other retaining glycosidase families. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.