Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil

Abstract

Phenol treatment was applied for isolation of rare Actinomycetes using 25 soil samples collected from Pulau Seribu, Tanjung Redep, Manokwari, and Halmahera. The samples were air-dried and suspended in 1.5 % (w/v) o phenol solution at 30 C for 30 minutes, and subsequently cultured on plates of humic acid-vitamin agar (HVA) -1 -1 medium supplemented with cycloheximide (50 µg mL) and nystatin (50 µg mL). A total of 61 isolates were obtained and the most dominant isolates were not Streptomyces (only 24.6%), whereas other genera such as Micromonospora, Actinomadura, Microbispora and Polymorphospora were isolated with ratios of 49.2%, 13.1%, 9.8%, and 3.3%, respectively. Perlakuan phenol diterapkan untuk mengisolasi Actinomycetes selain dari Streptomyces dari 25 sampel tanah yang diperoleh dari Pulau Seribu, Tanjung Redep, Manokwari, dan Halmahera. Sampel dikering anginkan o dan disuspensi dalam 1,5% larutan fenol pada suhu 30 C selama 30 menit, kemudian dikultur pada media asam -1 -1 humat-vitamin agar (HVA) yang dilengkapi dengan cycloheximide (50 µg mL) dan nistatin (50 µg mL). Sebanyak 61 isolat diperoleh dengan komposisi: Streptomyces hanya 24,6%, sedangkan marga lain seperti Micromonospora, Actinomadura, Microbispora dan Polymorphospora diperoleh masing-masing dengan rasio 49,2%, 13,1%, 9,8%, dan 3,3%. Kata kunci: Actinomycetes, metode isolasi selektif, perlakuan fenol, sampel tanah