Mulberry Leaves Extract Ameliorates Lipid Profile, Oxidative Stress and Aortic Histopathological Features In Dyslipidemic Rats Induced by A High-Fat Diet

Abstract

Coronary heart disease (CHD) are regarded as the crucial factors associated with dyslipidemia. Mulberry leaves are composed of several bioactive chemicals, such as phenols, flavonoids, anthocyanins, alkaloids and terpenoids. The effects of mulberry leaves on dyslipidemia have been considered in scientific research. This study determined the effect of mulberry leaf extract on TG, LDL-C, ox-LDL serum levels, and aortic histopathological features. A randomized controlled study was carried out on rats at the Center for Food and Nutrition Studies, Universitas Gadjah Mada, Yogyakarta, Indonesia. Twenty four Sprague Dawley (SD) rats were divided into normal control (n = 6) and dyslipidemia (Dys) (n = 18) groups. Rats in the Dys group were induced by a high-fat-diet (HFD). The Rats were categorized into 4 groups: the normal control group (N), negative control group (Dys), HFD+Simvastatin 0.18 mg/200gBW positive control (PC), and HFD+600 mg/kgBW mulberry leaf extract (MLE). Treatments were given daily for 8 weeks. The lipid profile parameters (TG, LDL, and HDL), ox-LDL levels and aortic histopathological features were evaluated using standard procedures. The results showed that MLE gave a significant differences in TG, LDL-C, HDL-C and ox-LDL levels in all groups (p<0,001). The mean changed TG, LDL-C, HDL-C and ox-LDL observed were 60.35 mg/dL, 49.43 mg/dL, 47.05 mg/dL, and 119.9 pg/mL, respectively. The aortic histopathological features indicated a statistically significant variance between the MLE group and the Dys group (p<0.001). The findings revealed that mulberry leaf extract significantly improved lipid profile, ox-LDL levels and aortic histopathological features.