Objective: To explore the liver-toxic fraction in Rhigoma of Dioscorea bulbifera. Method: The rats were randomized into four groups: control group (20%PVP-water), T001(10% total methanol extraction), F002(5% chloroform fraction) and P003(5% methanol fraction). Direct bilirubin (DBiI) and Glutamic-pyruvic transaminase (GPT) were examined, and liver index was measured. The histological and morphological observations were performed with optical and electrical microscope. Result: T001 and F002 showed significant liver toxicity. Conclusion: The chloroform fraction was the liver-toxic fraction of D. bulbifera.
Tan, X. Q., Ruan, J. L., Chen, H. S., & Wang, J. Y. (2003). Studies on liver-toxicity in Rhigoma of Dioscorea bulbifera. Zhongguo Zhongyao Zazhi, 28(7), 663.