Primary structure and functional expression from cDN A of the cardiac ryanodine receptor/calcium release channel

273Citations
Citations of this article
61Readers
Mendeley users who have this article in their library.

Abstract

The sequence of 4968 (or 4976 with an insertion) amino acids composing the ryanodine receptor from rabbit cardiac sarcoplasmic reticulum has been deduced by cloning and sequencing the cDNA. This protein is homologous in amino acid sequence and shares characteristic structural features with the skeletal muscle ryanodine receptor. Xenopus oocytes injected with mRNA derived from the cardiac ryanodine receptor cDNA exhibit Ca2+-dependent Cl- current in response to caffeine, which indicates the formation of functional calcium release channels. RNA blot hybridization analysis with a probe specific for the cardiac ryanodine receptor mRNA shows that the stomach and brain contain a hybridizable RNA species with a size similar to that of the cardiac mRNA. This result, in conjunction with cloning and analysis of partial cDNA sequences, suggests that the brain contains a cardiac type of ryanodine receptor mRNA. © 1990.

Cite

CITATION STYLE

APA

Nakai, J., Imagawa, T., Hakamata, Y., Shigekawa, M., Takeshima, H., & Numa, S. (1990). Primary structure and functional expression from cDN A of the cardiac ryanodine receptor/calcium release channel. FEBS Letters, 271(1–2), 169–177. https://doi.org/10.1016/0014-5793(90)80399-4

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free