Endogenous carbohydrate-binding proteins in Pneumocystis carinii

Abstract

By using biotinylated neoglycoproteins, the in situ occurrence of endogenous carbohydrate-binding proteins (lectins) in Pneumocystis carinii has been glycohistochemically demonstrated in lung tissue specimens from acquired immunodeficiency syndrome patients with P. carinii pneumonia. While the parasite possessed only a weak to moderate density of receptors for L-fucose and N-acetylated amino sugars, a strong specific binding of β-D-galactoside and D-mannoside neoglycoproteins was observed on the cyst surface and within intracystic bodies. It is suggested that these endogenous lectins may be involved in the adhesion of P. carinii to the target.