Nigrosporins A and B, new phytotoxic and antibacterial metabolites produced by a fungus nigrospora oryzae

Abstract

Nigrosporin A and B, two new phytotoxic and antibacterial metabolites were isolated from a culture filtrate of Nigrospora oryzae. The active principles were absorbed on XAD-2 resin and purified by successive ODS-HPLC. The structures were identified by spectroscopic and derivatization analysis as naphthoquinone derivatives. The substances showed phytotoxic activities, such as root elongation inhibition, necrotic effects, oxygen evolution inhibition, starch synthesis inhibition, and CO2fixation inhibition at concentrations of 10–100 ppm. They also showed growth inhibition activity against Bacillus subtilis in a disc diffusion assay as well as when compared with streptomycin. © 1997, Taylor & Francis Group, LLC. All rights reserved.