Standardization and comparative evaluation of phytochemical content and antioxidant activity of Alocasia indica and Tephrosia purpurea

Abstract

Extracts of leaves of Tephrosia purpurea and tuber of Alocasia indica were used as a traditional medicine in India for the management of various diseases, including rheumatic arthritis, hepatoprotective activity and antifungal properties.  The objective of the work is to standardize the phytochemical constituent and comparatively analyze the anti-oxidant activity of Alocasia indica and Tephrosia purpurea. Leaves ethanolic extract of Tephrosia purpurea and tuber ethanolic extract of Alocasia indicia Schott. were evaluated for antioxidant activity using 1, -diphenyl-2-picryl hydrazyl (DPPH) assay. Phenolic content was estimated by using Folin-Ciocalteu's reagent. Total flavonoid content was measured for Alocasia indica and Tephrosia purpurea by the aluminum chloride colorimetric assay. Furthermore, Albino mice were used to test the acute toxicity of the plant extracts.  The standardization of plant extracts demonstrated lesser amount of oil-soluble contents is present in both the plants. The total flavonoid contents were 76.93±1.41 and 107.44±1.89 mg QE/g while the total phenolic contents were 512.33±1.52 and 595.66±2.51 mg GAE/g for A. Indica and T. Perpurea, respectively. The antioxidant assay illustrated, significant DPPH radical scavenging potency of plant extracts.