ASSESSMENT OF TOTAL PHENOLIC, FLAVONOID CONTENT AND IN VITRO ANTIOXIDANT PROPERTIES OF ALCHEMILLIA VULGARIS (LADY'S MANTLE)

Abstract

The current study was designed to evaluate the phytochemical profile and biological properties (antioxidant activity) of methanolic, ethyl-acetate and petroleum extracts of Alchemilla vulgaris which was traditionally used to alleviate and treat many diseases. Total phenolic content found in methanolic and ethyl acetate extract of Alchemillia vulgaris were 547.333 and 386.86 respectively. The phenolic content with respect to gallic acid was found to be 547.333 and 386.86 (μg gallic acid equivalent/ml of extract) for ethyl acetate and methanol extract. The flavonoid content was found to be as: 275.00 and 153.33 (μg rutin equivalents/ml of extract) in ethyl acetate and methanol extract. Free radicals are concerned in many disorders like neurodegenerative disease; cancer and AIDS. Antioxidants during their scavenging power are helpful for the management of those diseases. DPPH stable free radical method is an easy, rapid and receptive way tosurvey the antioxidant activity of a precise compound or plant extracts. IC5 0of the standard compounds, ascorbic acid was 8.59μg/ml. The uppermost radical scavenging activity was showed by ethyl acetate and methanolic extracts of Alchemillia vulgaris IC50= 66.71 and 23.47μg/ml respectively. The ethyl acetate extract has shown significantly low anti-radical activity compared to the methanolic extract. The results indicate that the extract reduces the radicals to the corresponding hydrazine when it reacts with the hydrogen donors in the antioxidant principle. The superior amount of phenolic compounds leads to further potent radical scavenging result as shown by Alchemillia vulgaris leaves extract.