Nutritional Composition and In Vitro Antioxidant Activities of Seed Kernel and Seed Oil of Balanites roxburghii: An Underutilized Species

Abstract

The seed kernel of Balanites roxburghii Planch., an underutilized species, yields a substantial amount of oil and has an impressive fatty acid profile. Both seed cake and seed oil have the potential to be rich nutritive sources. With this view, in the present study, nutritive profiling of seed kernel and seed oil including HPLC quantification of tocopherols and phytosterols has been done. Further, the total phenolic content and antioxidant activities of seed kernel and oil have also been analyzed. The seed kernel showed good proximate composition with 43.20% of oil and 10.96% of protein with a good amount of macro- and microelements. The seed oil possesses 5.36 mg/kg of carotenoids and 7.29, 1.79, and 0.72 mg/100 g of α, β + γ (together), and δ-tocopherols, respectively. The β-Sitosterol is the major phytosterol in the oil with 126.90 mg/100 g oil followed by stigmasterol and campesterol together (40.78 mg/100 g oil). It is also rich in squalene (17.45 mg/100 g oil). Oleic acid (56.38%), linoleic acid (18.77%), and palmitic acid (17.79%) were found to be the major fatty acids. Seed cake and seed oil possess significant antioxidant activities with 2.72 mg Gallic acid equivalent (GAE)/g and 8.90 mg GAE/100 g total phenolic content, respectively. Even though seed kernels contain some amount of anti-nutritional factors, they can be minimized by practicing appropriate food processing techniques. Considering all these facts, such as availability, good quality oil, proteins, and elemental composition, seeds of B. roxburghii could be considered a reliable food source to increase the food base of people and to get a variety of nutrients.