Background: Methotrexate targets dihydrofolate reductase (DHFR) and thymidylate synthase (TYMS) to prevent cancer growth, and increases DHFR expression when applied. Results: TYMS resistant to methotrexate inhibits the methotrexate induced increase in DHFR expression. Conclusion: Thymidine synthesis regulates post-transcriptional expression of DHFR and TYMS. Significance: Methotrexate-resistant DHFR and TYMS can be used to regulate cis and trans transgene in primary T cells.
CITATION STYLE
Rushworth, D., Mathews, A., Alpert, A., & Cooper, L. J. N. (2015). Dihydrofolate reductase and thymidylate synthase transgenes resistant to methotrexate interact to permit novel transgene regulation. Journal of Biological Chemistry, 290(38), 22970–22976. https://doi.org/10.1074/jbc.C115.671123
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