Determination of gallic acid and rutin in extracts Cassia alata and Andrographis paniculata

Abstract

This study determines gallic acid and rutin in ethanol, methanol, and acetic acid extracts of Cassia alata and Andrographis paniculata. A preliminary study using thin layer chromatography and 0.2% DPPH (2, 2-diphenyl-1-picrylhydrazyl) spraying found that gallic acid was present in all three solvent extracts. Antioxidant components of all extractions were then analysed using high performance liquid chromatography with a C18 reverse-phase column at a flow rate of 0.50 ml/min and 25 °C. The results showed high components of gallic acid and rutin in all extracts of C. alata. The amounts of gallic acid found in C. alata extracted with ethanol, methanol, and acetic acid were 366, 410, and 258 mg/g crude extract, respectively. Amounts of gallic acid found in A. paniculata extracted with ethanol, methanol, and acetic acid were 309.4, 351.2, and 309.1 mg/g crude extract, respectively. Rutin concentrations found in C. alata extracted with ethanol, methanol and acetic acid were 194.4, 193.6, and 120.0 mg/g crude extract, respectively. The highest gallic acid level was found in C. alata extracted with methanol, while the highest rutin levels were found in C. alata extracted with ethanol and methanol.