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Carbohydrate gel electrophoresis.

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Abstract

Polysaccharide analysis using carbohydrate gel electrophoresis (PACE) relies on derivatization of the reducing ends of sugars with a fluorophore, followed by electrophoresis under optimized conditions in polyacrylamide gels. PACE is a sensitive and simple tool for studying polysaccharide structure or quantity and also has applications in the investigation of enzyme specificity.

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Goubet, F., Dupree, P., & Johansen, K. S. (2011). Carbohydrate gel electrophoresis. Methods in Molecular Biology (Clifton, N.J.), 715, 81–92. https://doi.org/10.1007/978-1-61779-008-9_5

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