Actinomycetospora iriomotensis sp. nov., a novel actinomycete isolated from a lichen sample

Abstract

An actinomycete strain, IR73-Li102T, was isolated from a lichen sample obtained from Iriomote Island, Japan, and subsequently characterized using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain IR73-Li102Thad the highest sequence similarities with Actinomycetospora chiangmaiensis YIM 0006T(98.3%), A. corticola 014-5T(98.1%) and A. rishiriensis RI109-Li102 T (98.0%). However, DNA-DNA hybridization assays, as well as physiological and biochemical analyzes, showed that strain IR73-Li102T could be clearly differentiated from its closest phylogenetic relatives. The strain contained meso-diaminopimelic acid, and arabinose and galactose were present in whole-cell hydrolysates. The predominant menaquinone was MK-8(H 4), and the diagnostic phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and diphosphatidylglycerol. The major cellular fatty acid was iso-C 16:0 (58%). The chemotaxonomic properties of strain IR73-Li102 T were consistent with those shared by members of the genus Actinomycetospora. On the basis of the phenotypic features and DNA-DNA hybridization data, strain IR73-Li102 T ( NBRC 106365T KCTC 19783T) represents a novel species of the genus Actinomycetospora, for which the name Actinomycetospora iriomotensis sp. nov. is proposed. © 2011 Japan Antibiotics Research Association. All rights reserved.