Effects of Acorns on Meat Quality and Lipid Metabolism-Related Gene Expression in Muscle Tissues of Yuxi Black Pigs

Abstract

Objectives: The aim of this study was to investigate the effects of acorn diets on the composition of fatty acid (FA) and the intramuscular fat (IMF) content in Yuxi black pigs. Methods: Ninety Yuxi black pigs with similar body weight (99.60 ± 2.32 kg) were randomly divided into five groups. The control group was fed a basal diet, and the AD20, AD30, AD40, and AD50 groups were fed experimental diets which contained 20%, 30%, 40%, and 50% acorns, respectively. The feeding experiment lasted for 120 days. Results: The results showed that compared with the control group, the content of SFA in longissimus dorsi and biceps femoris tissues in the AD30 group decreased by 8.57% and 20.10%, and the content of MUFA increased by 5.40% and 15.83%, respectively, while the PUFA content of biceps femoris increased by 5.40% (p < 0.05). Meanwhile, the IMF content of the AD30 group was significantly higher than that of the control group in the longissimus dorsi and biceps femoris. In addition, the mRNA expression levels of the ATGL, PPARγ, and FABP4 genes in longissimus dorsi (p < 0.05) were up-regulated, and HSL were down-regulated (p < 0.05) in the AD30 group. In the biceps femoris of the AD30 group, it was observed that the expression levels of the ACC and FAS genes were up-regulated (p < 0.05), while HSL and ATGL genes were down-regulated (p < 0.05). Conclusions: These results demonstrated that the addition of appropriate amounts of acorn to the diet (a 30% acorn diet) could improve the nutritional value of pork.