Relationship of interferon regulator factor 5 and interferon‑γ with missed abortion

Abstract

The aim of the present study was to reveal the association of missed abortion, a process integrated with the immune system, with interferon regulatory factor 5 (IRF5) and interferon-gamma (IFN-gamma), and to demonstrate the function of these molecules by examining their levels in decidual tissue. This prospective cohort study included 13 patients with no additional systemic disease, between 6 and 10 weeks of gestation with negative fetal heartbeat, and 11 patients between 6 and 10 weeks of gestation with positive heartbeat who presented for voluntary termination of pregnancy. In the fresh decidual tissue materials recovered after therapeutic curettage, IFN-gamma and IRF5 protein levels were determined by ELISA method and IFN-gamma and IRF5 gene expression levels by qPCR method. The mean IFN-gamma (86.5 vs. 27.3 pg/mg protein; P < 0.001) and IRF5 (2.0 vs. 1.5 ng/mg protein; P < 0.001) levels were significantly higher in pregnant women who had missed abortion compared to the voluntary abortion group. The increases in the mean IFN-gamma/GAPDH (3.5 vs. 1.5-fold increase; P < 0.001) and IRF5/GAPDH (3.9 vs. 1.4-fold increase; P < 0.001) gene expression levels were significantly higher in the tissues of pregnant women with missed abortion than in the voluntary abortion group. A threshold value of 45.2 pg/mg protein for IFN-gamma had a sensitivity of 100% and specificity of 100% in determination of missed abortion. The findings of present study revealed, to the best of our knowledge for the first time in the literature, that IFN-gamma and IRF5 may be associated with missed abortion, and that IFN-gamma and IRF5 protein levels and gene expression levels were significantly increased in the case of missed abortion. According to our findings, IFN-gamma and IRF5 play an important role in placental invasion and pregnancy and can be used as markers for endometrial implantation.