Effect of amino groups of mesoporous silica nanoparticles on CpG oligodexynucleotide delivery

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Abstract

© 2015 National Institute for Materials Science. In this study, we proposed to modify mesoporous silica nanoparticles (MSNs) with 3-aminopropyltriethoxysilane (NH < inf > 2 < /inf > -TES), aminoethylaminopropyltriethoxysilane (2NH < inf > 2 < /inf > -TES) and 3-[2-(2-aminoethylamino)ethylamino] propyl-trimethoxysilane (3NH < inf > 2 < /inf > -TES) for binding of cytosine-phosphate-guanosine oligodexynucleotides (CpG ODN), and investigated the effect of different amino groups of MSNs on the CpG ODN delivery. Serum stability, in vitro cytotoxicity, and cytokine interleukin-6 (IL-6) induction by MSN-NH < inf > 2 < /inf > /CpG, MSN-2NH < inf > 2 < /inf > /CpG and MSN-3NH < inf > 2 < /inf > /CpG complexes were investigated in detail. The results showed that three kinds of aminated-MSN-based CpG ODN delivery systems had no cytotoxicity to RAW264.7 cells, and binding of CpG ODN to MSN-NH < inf > 2 < /inf > , MSN-2NH < inf > 2 < /inf > and MSN-3NH < inf > 2 < /inf > nanoparticles enhanced the serum stability of CpG ODN due to protection by the nanoparticles. However, three aminated MSN-based CpG ODN delivery systems exhibited different CpG ODN delivery efficiency, and MSN-NH < inf > 2 < /inf > /CpG complexes had the highest ability to induce IL-6 secretion.

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Xu, Y., Claiden, P., Zhu, Y., Morita, H., & Hanagata, N. (2015). Effect of amino groups of mesoporous silica nanoparticles on CpG oligodexynucleotide delivery. Science and Technology of Advanced Materials, 16(4). https://doi.org/10.1088/1468-6996/16/4/045006

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