Effect of novel marine nutraceuticals on IL-1 -mediated TNF- release from UVB-irradiated human melanocyte-derived cells

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Abstract

UV-induced inflammation and reactive oxygen species formation are involved in the development of melanoma. Natural products like 5 β -scymnol and CO 2 -supercritical fluid extract (CO 2 -SFE) of mussel oil contain anti-inflammatory and antioxidant properties that may aid in reducing the deleterious effects of UV radiation. Therefore, their effect on the release of the proinflammatory cytokine, tumour necrosis factor- α (TNF- α ), from UVB-irradiated human melanocytic cells was examined. Human epidermal melanocytes (HEM) and MM96L melanoma cells were exposed to UVB radiation and IL-1 α . Cell viability and TNF- α levels were determined 24 hours after-irradiation while p38 mitogen-activated protein kinase (MAPK) activation was observed at 15 min after-irradiation. When α -tocopherol, CO 2 -SFE mussel oil, and 5β-scymnol were added to the UVB-irradiated HEM cells treated with IL-1 α , TNF- α levels fell by 53%, 65%, and 76%, respectively, while no inhibition was evident in MM96L cells. This effect was not due to inhibition of the intracellular p38 MAPK signalling pathway. These compounds may be useful in preventing inflammation-induced damage to normal melanocytes.

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Muthusamy, V., Hodges, L. D., MacRides, T. A., Boyle, G. M., & Piva, T. J. (2011). Effect of novel marine nutraceuticals on IL-1 -mediated TNF- release from UVB-irradiated human melanocyte-derived cells. Oxidative Medicine and Cellular Longevity. https://doi.org/10.1155/2011/728645

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