The effects of flash-annealing on glycerol kinase crystals

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Abstract

Reflection profiles from glycerol kinase crystals were analyzed to determine the effect of flash-cooling on mosaicity (eta) and peak intensity in order to reveal changes in mosaic domain structure and composition. The results showed that repeated flash-annealing causes a significant decrease in the averaged mosaicity along with an increase in the overall peak counts of reflections and an enhanced signal-to-noise ratio. Individual reflection-profile analysis revealed a mostly dual domain structure, showing the minimization of one domain as a result of flash-annealing.

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Vahedi-Faridi, A., Stojanoff, V., & Yeh, J. I. (2005). The effects of flash-annealing on glycerol kinase crystals. Acta Crystallographica Section D: Biological Crystallography, 61(7), 982–989. https://doi.org/10.1107/S0907444905012746

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