Efficient engineering of marker-free synthetic allotetraploids of Saccharomyces

18Citations
Citations of this article
72Readers
Mendeley users who have this article in their library.

Abstract

Saccharomyces interspecies hybrids are critical biocatalysts in the fermented beverage industry, including in the production of lager beers, Belgian ales, ciders, and cold-fermented wines. Current methods for making synthetic interspecies hybrids are cumbersome and/or require genome modifications. We have developed a simple, robust, and efficient method for generating allotetraploid strains of prototrophic Saccharomyces without sporulation or nuclear genome manipulation. S. cerevisiae × S. eubayanus, S. cerevisiae × S. kudriavzevii, and S. cerevisiae × S. uvarum designer hybrid strains were created as synthetic lager, Belgian, and cider strains, respectively. The ploidy and hybrid nature of the strains were confirmed using flow cytometry and PCR-RFLP analysis, respectively. This method provides an efficient means for producing novel synthetic hybrids for beverage and biofuel production, as well as for constructing tetraploids to be used for basic research in evolutionary genetics and genome stability.

Cite

CITATION STYLE

APA

Alexander, W. G., Peris, D., Pfannenstiel, B. T., Opulente, D. A., Kuang, M., & Hittinger, C. T. (2016). Efficient engineering of marker-free synthetic allotetraploids of Saccharomyces. Fungal Genetics and Biology, 89, 10–17. https://doi.org/10.1016/j.fgb.2015.11.002

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free