Electrochemical Immunosensor for Detection of IgY in Food and Food Supplements

  • Gaetani C
  • Ambrosi E
  • Ugo P
  • et al.
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Abstract

© 2017 by the authors. Immunoglobulin Y is a water-soluble protein present in high concentration in hen serum and egg yolk. IgY has applications in many fields, e.g., from food stuffto the mass production of antibodies. In this work, we have implemented an electrochemical immunosensor for IgY based on templated nanoelectrodes ensembles. IgY is captured by the templating polycarbonate and reacted with anti-IgY labeled with horseradish peroxidase. In the presence of H 2 O 2 and methylene blue as the redox mediator, an electrocatalytic current is generated which scales with IgY concentration in the sample. After optimizing the extracting procedure, the immunosensor was applied for analysis of fresh eggs and food integrators. The data obtained with the biosensor were validated by SDS-PAGE and Western blot measurements.

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Gaetani, C., Ambrosi, E., Ugo, P., & Moretto, L. (2017). Electrochemical Immunosensor for Detection of IgY in Food and Food Supplements. Chemosensors, 5(1), 10. https://doi.org/10.3390/chemosensors5010010

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