Enhancement of protein expression in insect cells by a lobster tropomyosin cDNA leader sequence

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Abstract

We describe a cis element that dramatically increases the expression levels of exogenous genes in baculovirus-infected insect cells. This 21 bp sequence element is derived from a 5′ untranslated leader sequence of a lobster tropomyosin cDNA (L21). By using a transfer vector carrying L21, the expression levels of tropomyosin and luciferase were 20- and seven-fold higher with L21 than without L21, respectively. L21 has both the Kozak sequence and the A-rich sequence found in the polyhedrin leader sequence. We assume that both sequence elements are essential for the enhancement of protein expression in the baculovirus-based expression system. © 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

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Sano, K. I., Maeda, K., Oki, M., & Maéda, Y. (2002). Enhancement of protein expression in insect cells by a lobster tropomyosin cDNA leader sequence. FEBS Letters, 532(1–2), 143–146. https://doi.org/10.1016/S0014-5793(02)03659-1

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