The ompX gene of Enterobacter aerogenes was cloned. Its overexpression induced a decrease in the major porin Omp36 production and consequently a β-lactam resistance was noted. Purified outer membrane protein X (OmpX) was reconstituted into artificial membranes and formed ion channels with a conductance of 20 pS in 1 M NaCl and a cationic selectivity. Both MarA expression and high osmolarity induced a noticeable increase of the OmpX synthesis in the E. aerogenes ATCC 13048 strain. In addition, OmpX synthesis increased under conditions in which the expression of the E. aerogenes major non-specific porins, Omp36 and Omp35, decreased. © 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
Dupont, M., Dé, E., Chollet, R., Chevalier, J., & Pagès, J. M. (2004). Enterobacter aerogenes OmpX, a cation-selective channel mar- and osmo-regulated. FEBS Letters, 569(1–3), 27–30. https://doi.org/10.1016/j.febslet.2004.05.047