Establishment of a reborn MMV-microarray technology: Realization of microbiome analysis and other hitherto inaccessible technologies

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Abstract

BACKGROUND: With the accelerating development of bioscience, the problem of research cost has become important. We previously devised and developed a novel concept microarray with manageable volumes (MMV) using a soft gel. It demonstrated the great potential of the MMV technology with the examples of 1024-parallel-cell culture and PCR experiments. However, its full potential failed to be expressed, owing to the nature of the material used for the MMV chip.<br /><br />RESULTS: In the present study, by developing plastic-based MMVs and associated technologies, we introduced novel technologies such as C2D2P (in which the cells in each well are converted from DNA to protein in 1024-parallel), NGS-non-dependent microbiome analysis, and other powerful applications.<br /><br />CONCLUSIONS: The reborn MMV-microarray technology has proven to be highly efficient and cost-effective (with approximately 100-fold cost reduction) and enables us to realize hitherto unattainable technologies.

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Sharma, H., Kinoshita, Y., Fujiu, S., Nomura, S., Sawada, M., Ahmed, S., … Nishigaki, K. (2014). Establishment of a reborn MMV-microarray technology: Realization of microbiome analysis and other hitherto inaccessible technologies. BMC Biotechnology, 14(1). https://doi.org/10.1186/1472-6750-14-78

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