Evaluation of dot-blot test for serological diagnosis of bovine brucellosis

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Abstract

The objective of this study was to standardize and validate the dot-blot test for the serological diagnosis of bovine brucellosis, compare the results with those found in the 2-mercaptoethanol (2-ME) and complement fixation test (CF), and estimate the relative sensitivity and specificity of the dot-blot compared to these tests. Fifty bovine blood serum samples were used for the test standardization, and 1315 samples were used for evaluation and comparison between the tests; the results were compared using the Kappa indicator. At the end of standardization, it was established as optimal for the antigen obtained from Brucella abortus B19 after passing through a microorganism rupture process, the blood serum samples diluted at 1:100, and the conjugate at 1:30,000. The comparison of the dot-blot results with 2-ME showed Kappa index of 0.9939, sensitivity of 99.48%, and specificity 99.91%, with CF, Kappa index of 0.8226, sensitivity 100% and specificity 95.32%. Using the combination of the test results 2-ME and CF to establish the true condition of the animal, the dot-blot showed relative sensitivity of 100%, and relative specificity of 99.91%. The evaluated test proved to be effective and reliable, besides being easy to handle and interpret the results.

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Bastos, C. R., Mathias, L. A., Jusi, M. M. G., Santos, R. F. dos, Silva, G. C. P. da, André, M. R., … Bürger, K. P. (2018). Evaluation of dot-blot test for serological diagnosis of bovine brucellosis. Brazilian Journal of Microbiology, 49(3), 564–568. https://doi.org/10.1016/j.bjm.2017.10.002

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