Next-generation sequencing (NGS) technologies provide a revolutionary tool with numerous applications in transcriptome studies. The power of NGS technologies to address diverse biological questions has already been proved in many studies. One of the most important applications of NGS is the sequencing and characterization of transcriptome of a non-model species using RNA-seq. This application of NGS technologies can be used to dissect the complete expressed gene content of an organism. In this article, I illustrate the use of NGS technologies in transcriptome characterization of a non-model species taking example of chickpea from our recent studies.
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