Many Nigerian Plants have been hypothesized to have anticancer potentials.(1) However, six of such plants were selected to evaluate the effects of their crude, hexane, chloroform, ethylacetate, detanninfied and tannin fractions for brineshrimp lethality assay and the most cytotoxic fractions of each plant were further tested on gene expressions of p53 and retinoblastoma (Rb) in human breast cancer cell line (MCF-7). Gladiolus psittacinus (Gps), Icacina trichantha (Itr), Spilanthes filicaulis (Sfi), Curculigo pilosa (Cpi), Anthocleist adjalonensis (Adj), and Tapinanthus bangwensis (Tba) medicinal plants were selected. Crude extracts of 80% aqueous ethanol macerated plant materials were fractionated into hexane, chloroform and ethylacetate fractions. 5 g each of concentrated crude extracts were dissolved in 20 ml deionised water and 70 ml of the organic solvent (n-hexane) was added and the immiscible mixture was transferred into a separatory funnel. The mixture was allowed to phase separate to aqueous and organic fractions. The aqueous fractions gave detanninfied and tannin fractions. The 36 panel of plant fractions produced from all the plants were used for the study. Hexane fraction of Spilanthes filicaulis (Sfi-HF) showed the highest cytotoxic effect (LC50 21.30 mug/ml) on brineshrimps showing a low signal of p53 gene expression but a high intensity of retinoblastoma (Rb) gene expression in MCF-7 cell lines. Crude extract of Gladiolus psittacinus (Gps-CE) showed a significant (P < 0.05) increase in p53 gene expression in comparison with the control group and a high intensity of Rb gene expression. Results demonstrate the modulatory potentials of Sfi-HF and Gps-CE on p53 and Rb gene expressions in MCF-7 breast cancer cell lines suggesting a possible mode of action of Sfi-HF and Gps-CE amongst a panel of 36 extract fractions.
Samuel, T. A., James, A. B., Oshodi, T. A., Odii, U. O., Chidume, I., Makinde, O. E., … Magbagbeola, O. A. (2016). Evaluation of retinoblastoma (Rb) and protein-53 (p53) gene expression levels in breast cancer cell lines (MCF-7) induced with some selected cytotoxic plants. Journal of Pharmacognosy and Phytotherapy, 5(7), 120–126. https://doi.org/10.5897/jpp2013.0270