© 2017, European Centre for Disease Prevention and Control (ECDC). All rights reserved. We evaluated a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (LA-MRSA) in samples collected from pigs and the environment inside pig stables in Denmark and Norway. Selective enrichment in tryptic soy broth containing cefoxitin and aztreonam led to a high ratio of false-negative results (26%; 57/221). On this basis, we recommend reconsidering the use of selective enrichment for detection of LA-MRSA in animal and environmental samples.
Larsen, J., Sunde, M., Islam, M. Z., Urdahl, A. M., Barstad, A. S., Larsen, A. R., … Angen. (2017). Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016. Eurosurveillance, 22(28). https://doi.org/10.2807/1560-7917.ES.2017.22.28.30573