Evidence of finely tuned expression of DNA polymerase β in vivo using transgenic mice

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Abstract

DNA polymerase β (Pol β) is an error-prone repair DNA polymerase that has been shown to create genetic instability and tumorigenesis when overexpressed by only 2-fold in cells, suggesting that a rigorous regulation of its expression may be essential in vivo. To address this question, we have generated mice which express a transgene (Tg) bearing the Pol β cDNA under the control of the ubiquitous promoter of the mouse H-2K gene from the major histocompatibility complex. These mice express the Tg only in thymus, an organ which normally contains the most abundant endogenous Pol β mRNA and protein, supporting the idea of a tight regulation of Pol β in vivo. Furthermore, we found no tumor incidence, suggesting that the single Pol β overexpression event is not sufficient to initiate tumorigenesis in vivo. © 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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Bergoglio, V., Fréchet, M., Philippe, M., Bieth, A., Mercier, P., Morello, D., … Cazaux, C. (2004). Evidence of finely tuned expression of DNA polymerase β in vivo using transgenic mice. FEBS Letters, 566(1–3), 147–150. https://doi.org/10.1016/j.febslet.2004.04.020

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