A beginner's guide to mass spectrometry—based proteomics

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Abstract

Mass spectrometry (MS)-based proteomics is the most comprehensive approach for the quantitative profiling of proteins, their interactions and modifications. It is a challenging topic as a firm grasp requires expertise in biochemistry for sample preparation, analytical chemistry for instrumentation and computational biology for data analysis. In this short guide, we highlight the various components of a mass spectrometer, the sample preparation process for conversion of proteins into peptides, and quantification and analysis strategies. The advancing technology of MS-based proteomics now opens up opportunities in clinical applications and single-cell analysis.

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Sinha, A., & Mann, M. (2020). A beginner’s guide to mass spectrometry—based proteomics. Biochemist, 42(5), 64–69. https://doi.org/10.1042/bio20200057

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