Evaluation of Antibacterial Activity of Selenium and Copper Oxide Nanoparticles Synthesized by Hibiscus sabdariffa L. Extract

Abstract

The aim of the present study is to synthesize selenium (SeNPs) and copper oxide (CuONPs) nanoparticles utilizing aqueous extract of Hibiscus sabdariffa L. as a reducing agent. The green synthesized Hibiscus sabdariffa L. selenium and copper oxide nanoparticles were identified by color change. The characterization of SeNPs and CuONPs was achieved by Ultraviolet-visible (UV-VIS) spectroscopy, Field Emission Scanning electron microscope (FESEM), Atomic force microscope (AFM), X-Ray diffraction analysis (XRD) and transform infrared spectroscopy (FTIR). These tests are utilized for detecting stability, morphology, size, crystalline nature and functional groups on nanoparticles surface prepared. The outcomes revealed appearance of the prick-red and green color, indicating a specific color of selenium and copper oxide nanoparticles respectively. It also disclosed that UV-VIS spectroscopy indicated band absorbance at 234 and 274 nanometer of intense surface Plasmon resonance, manifesting the formation and stability of prepared SeNPs and CuONPs. The FESEM image displayed mulit-shapes between spherical and vaulted for selenium and copper oxide nanosized. XRD at 2 theta revealed crystalline selenium and copper oxide nanoparticles, with (54.92-57.79) nm average size. FTIR revealed the presence of functional groups of the plant that act as stabilizing and reducing agents. The antibacterial activity of synthesized nanoparticles was studied against five different bacteria causing food spoilage.