Skip to main content

Automated 3D gene position analysis using a customized Imaris plugin: XTFISHInsideNucleus

4Citations
Citations of this article
10Readers
Mendeley users who have this article in their library.
Get full text

Abstract

Fluorescence in situ hybridization (FISH) is commonly used to visualize chromosomal regions or genomic loci within the nucleus, and can largely contribute to unraveling the link between structure and function in the nucleus. Three-dimensional (3D) analyses are required to best capture the nucleus’ organizing principles, but the experimental setup and computational analyses are far from trivial. Here, we present a robust workflow for 3D FISH against repeats and single copy loci in embedded intact nuclei from Arabidopsis leaves. We then describe in detail the image acquisition, subsequent image deconvolution before 3D image processing, and the image reconstruction. We developed an automated batch image processing pipeline using a customized, open source plugin implemented in the Imaris environment.

Cite

CITATION STYLE

APA

Ashenafi, M. S., & Baroux, C. (2018). Automated 3D gene position analysis using a customized Imaris plugin: XTFISHInsideNucleus. In Methods in Molecular Biology (Vol. 1675, pp. 591–613). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7318-7_32

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free