Molecular analysis of the c-myc locus in normal tissue and in avian leukosis virus-induced lymphomas

  • Neel B
  • Gasic G
  • Rogler C
  • et al.
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Abstract

We isolated molecular clones of the provirus-host cell junctions (tumor junction fragments) from two avian leukosis virus-induced lymphomas and compared the structures of these clones with a clone of the normal c-myc gene. Restriction mapping and DNA sequencing demonstrated that normal proviral integration events occurred adjacent to c-myc in both tumors, without gross structural alteration of c-myc. The right long terminal repeat of an avian leukosis virus provirus is integrated upstream from the bulk of the c-myc coding sequences and oriented such that transcription can initiate within the long terminal repeat and proceed downstream into c-myc. A comparison of a tumor junction fragment with the v-myc gene showed that there are two regions of v-myc-related sequences (which are probably exons) separated by 1 kilobase of sequences unrelated to v-myc (probably an intron). A DNA sequence analysis of the tumor junction fragments suggested that integration had occurred in exons adjacent to splice donor sites. This suggests that there are additional exons and introns in c-myc. Based on these findings, a model is proposed for the genesis of the tumor-specific RNAs containing viral-5' and c-myc information in avian leukosis virus-induced lymphomas.

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Neel, B. G., Gasic, G. P., Rogler, C. E., Skalka, A. M., Ju, G., Hishinuma, F., … Hayward, W. S. (1982). Molecular analysis of the c-myc locus in normal tissue and in avian leukosis virus-induced lymphomas. Journal of Virology, 44(1), 158–166. https://doi.org/10.1128/jvi.44.1.158-166.1982

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