Uso de polímeros em formulações para preservação de Pectobacterium atrosepticum e Ralstonia solanacearum

Abstract

The survival of Pectobacterium atrosepticum and Ralstonia solanacearum was evaluated in biopolymer carrier xanthan gum (GX) and xanthan gum together with polymer synthetic polyvinylpyrrolidone (PVP). The cultures after growing up to 1011 ufc mL-1 were injected into the plolymers carriers. The survival of the bacteria was evaluated at room temperature, refrigerator (4°C) and freezer (-20°C) for 36 months. The evaluation of viability of the preserved bacteria was carried through seriate dilutions using drop method. The pathogenicity was observed in plant hosts and through baits. The results allow observing that the concentration of viable cells decreased along the time mainting to 103 and 104 ufc mL-1 for three years with exception, of the formulation to the base of GX and storage the -20°C, that was inefficient for preservation of both the tested bacteria. The polymers applied persevering and mainting the characteristic biochemical and physiological of the bacteria showing efficient to preservation.