Genetic recombination in Coprinus. IV. A kinetic study of the temperature effect on recombination frequency

Abstract

At the restrictive conditions (35°C under continuous light) Coprinus lagopus is unable to initiate premeiotic S phase which takes place normally within 8 to 10 hr of karyogamy. A shift up to the restrictive conditions causes an arrest of the basidiocarps at this critical stage. A prolonged arrest causes a reversal to mitosis (LU 1974). Incubation of basidiocarps at the restrictive conditions before this critical stage causes no increase in recombination frequency (R.F.) in the loci studied. An arrest of 4 hr at the critical stage still causes no R.F. increase, but 12 to 13 hr and 18 to 19 hr arrests cause increases of 50% and 90% over the controls, respectively. Thus R.F. can be increased even before the cells are fully committed to meiosis. A 3 hr heat treatment at the beginning of S phase (or 8 hr before karyogamy) also causes some (30%) increase in R.F. while the same treatment at late S phase (or 3 hr before karyogamy) causes a substantial (164%) increase in R.F. over the controls. A 3 hr heat treatment before S phase causes no increase in R.F. Pachytene is also responsive to temperature treatments (LU 1969). The maximum R.F. increase is 100% by heat and 220% by cold treatment. The shortest time that can cause the maximum increase in recombination by high temperature is 3 hr and that by cold treatment is 7 hr. These durations are correlated with the length of the pachytene stage under the treatment conditions. The kinetic data show that the increase in R.F. caused by high and low temperatures follows two hit kinetics and their rate of increase is almost identical. The higher increase in R.F. by low temperature can be attributed to the increased duration of pachytene and therefore R.F. is a function of time. The longer the homologous chromosomes are held together, the higher the recombination frequency.