Phenotypic and genotypic profile of pyrethroid resistance in populations of the mosquito aedes aegypti from Goiânia, Central West Brazil

Abstract

Introduction: The mosquito Aedes aegypti has evolved resistance to pyrethroid insecticides. The present study evaluated Ae. aegypti from Goiânia for the resistant phenotype and for mutations associated with resistance. Methods: Insecticide dose-response bioassays were conducted on mosquitoes descended from fi eld-collected eggs, and polymerase chain reaction (PCR) was used to genotype 90 individuals at sites implicated in pyrethroid resistance. Results: All mosquito populations displayed high levels of resistance to deltamethrin, as well as high frequencies of the 1016Ilekdr and 1534Cyskdr mutations. Conclusions: Aedes aegypti populations in the Goiânia area are highly resistant to deltamethrin, presumably due to high frequencies of kdr (knockdown-resistance) mutations.